Troubleshooting TLC

The information provided above (including the procedure page) may give the impression that TLC is a relatively simple procedure. However, consider the experience of conducting a TLC for the first time, where one might observe spots scattered throughout and blurred, streaked areas. Like any technique, improvement comes with practice. Below are examples of typical issues … Read more

Thin Layer Chromatography (TLC)

Thin layer chromatography (TLC) is a technique utilized in chromatography for the separation of mixtures. This process is conducted on a substrate made of glass, plastic, or aluminum foil, which is covered with a thin layer of adsorbent material, typically silica gel, aluminum oxide, or cellulose (blotter paper). The adsorbent layer is referred to as … Read more